› 4jq6

X-ray diffraction
2.31Å resolution

Crystal structure of blue light-absorbing proteorhodopsin from Med12 at 2.3 Angstrom

Released:
DOI: 10.2210/pdb4jq6/pdb
PDB entry 4jq6 coloured by chain, front view.
PDB entry 4jq6 coloured by chain, side view.
PDB entry 4jq6 coloured by chain, top view.
Source organism: uncultured bacterium
Primary publication:
Cross-protomer interaction with the photoactive site in oligomeric proteorhodopsin complexes.
Ran T, Ozorowski G, Gao Y, Sineshchekov OA, Wang W, Spudich JL, Luecke H
Acta Crystallogr D Biol Crystallogr 69 1965-80 (2013)
PMID: 24100316

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175656 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Proteorhodopsin Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 235 amino acids
Theoretical weight: 25.89 KDa
Source organism: uncultured bacterium
Expression system: Escherichia coli
UniProt:
  • Canonical: Q4PP54 (Residues: 1-235; Coverage: 100%)
Sequence domains: Bacteriorhodopsin-like protein
Structure domains: Rhodopsin 7-helix transmembrane proteins

Ligands and Environments

2 bound ligands:
Ligand RET 3 x RET
Ligand LI1 2 x LI1
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Unit cell:
a: 87.267Å b: 101.952Å c: 87.357Å
α: 90° β: 119.52° γ: 90°
R-values:
R R work R free
0.211 0.208 0.266
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Microbial Rhodopsins: Diversity, Mechanisms, and Optogenetic Applications.
Govorunova et al. (2017)
6 more

20 mentions without citation

Multiscale Simulations of Biological Membranes: The Challenge To Understand Biological Phenomena in a Living Substance.
Enkavi et al. (2019)
19 more