› 4jlx

X-ray diffraction
2Å resolution

Structure of porcine cyclic GMP-AMP synthase (cGAS)

Released:
DOI: 10.2210/pdb4jlx/pdb
PDB entry 4jlx coloured by chain, front view.
PDB entry 4jlx coloured by chain, side view.
PDB entry 4jlx coloured by chain, top view.
Source organism: Sus scrofa
Primary publication:
Structural mechanism of cytosolic DNA sensing by cGAS.
Civril F, Deimling T, de Oliveira Mann CC, Ablasser A, Moldt M, Witte G, Hornung V, Hopfner KP
Nature 498 332-7 (2013)
PMID: 23722159

Function and Biology Details

Reaction catalysed: 
(1a) ATP + GTP = pppG-P(2'-5')A + diphosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125075 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cyclic GMP-AMP synthase Chain: A
Molecule details ›
Chain: A
Length: 366 amino acids
Theoretical weight: 42.47 KDa
Source organism: Sus scrofa
Expression system: Escherichia coli
UniProt:
  • Canonical: I3LM39 (Residues: 135-267, 268-495; Coverage: 73%)
Gene names: CGAS, MB21D1
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand ZN 1 x ZN
Ligand MLI 3 x MLI
Ligand GOL 1 x GOL
Ligand PG4 2 x PG4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: C2221
Unit cell:
a: 47.402Å b: 118.014Å c: 142.599Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.188 0.215
Expression system: Escherichia coli

Quick links

Citations

191 review citations

Regulation and function of the cGAS-STING pathway of cytosolic DNA sensing.
Chen et al. (2016)
190 more

8 mentions without citation

Regulation of cGAS-Mediated Immune Responses and Immunotherapy.
Saeed et al. (2020)
7 more