› 4jfc

X-ray diffraction
2.25Å resolution

Crystal structure of a enoyl-CoA hydratase from Polaromonas sp. JS666

Released:
DOI: 10.2210/pdb4jfc/pdb
PDB entry 4jfc coloured by chain, front view.
PDB entry 4jfc coloured by chain, side view.
PDB entry 4jfc coloured by chain, top view.
Source organism: Polaromonas sp. JS666
Entry authors: Kumaran D, Chamala S, Evans B, Foti R, Gizzi A, Hillerich B, Kar A, Lafleur J, Seidel R, Villigas G, Zencheck W, Al Obaidi N, Almo SC, Swaminathan S, New York Structural Genomics Research Consortium (NYSGRC)

Function and Biology Details

Reaction catalysed: 
(3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171469 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Enoyl-CoA hydratase Chain: A
Molecule details ›
Chain: A
Length: 281 amino acids
Theoretical weight: 29.94 KDa
Source organism: Polaromonas sp. JS666
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q129C0 (Residues: 1-259; Coverage: 100%)
Gene name: Bpro_2958
Sequence domains: Enoyl-CoA hydratase/isomerase
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand GOL 1 x GOL
1 modified residue:
Ligand MSE 5 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P63
Unit cell:
a: 111.182Å b: 111.182Å c: 41.17Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.17 0.168 0.211
Expression system: Escherichia coli BL21(DE3)

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