› 4jar

X-ray diffraction
1.98Å resolution

Crystal structure of mycobacterium tuberculosis pks11 in complex with polyketide intermediates and evidence that it synthesize ALKYLPYRONES

Released:
DOI: 10.2210/pdb4jar/pdb
PDB entry 4jar coloured by chain, front view.
PDB entry 4jar coloured by chain, side view.
PDB entry 4jar coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
Crystal structure of Mycobacterium tuberculosis polyketide synthase 11 (PKS11) reveals intermediates in the synthesis of methyl-branched alkylpyrones.
Gokulan K, O'Leary SE, Russell WK, Russell DH, Lalgondar M, Begley TP, Ioerger TR, Sacchettini JC
J Biol Chem 288 16484-16494 (2013)
PMID: 23615910

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161977 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Methyl-branched alkylpyrone synthesis polyketide synthase-like Pks11 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 353 amino acids
Theoretical weight: 37.68 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Mycolicibacterium smegmatis
UniProt:
  • Canonical: P9WPF3 (Residues: 1-353; Coverage: 100%)
Gene names: Rv1665, pks11
Sequence domains:
Structure domains: Peroxisomal Thiolase; Chain A, domain 1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P21
Unit cell:
a: 72.598Å b: 48.521Å c: 195.234Å
α: 90° β: 98.41° γ: 90°
R-values:
R R work R free
0.27 0.268 0.314
Expression system: Mycolicibacterium smegmatis

Quick links

Citations

8 review citations

Type III Polyketide Synthases: Functional Classification and Phylogenomics.
Shimizu et al. (2017)
7 more

1 mention without citation

Crystal structure of Mycobacterium tuberculosis polyketide synthase 11 (PKS11) reveals intermediates in the synthesis of methyl-branched alkylpyrones.
Gokulan et al. (2013)