› 4j72

X-ray diffraction
3.3Å resolution

Crystal Structure of polyprenyl-phosphate N-acetyl hexosamine 1-phosphate transferase

Released:
DOI: 10.2210/pdb4j72/pdb
PDB entry 4j72 coloured by chain, front view.
PDB entry 4j72 coloured by chain, side view.
PDB entry 4j72 coloured by chain, top view.
Source organism: Aquifex aeolicus
Primary publication:
Crystal structure of MraY, an essential membrane enzyme for bacterial cell wall synthesis.
Chung BC, Zhao J, Gillespie RA, Kwon DY, Guan Z, Hong J, Zhou P, Lee SY
Science 341 1012-1016 (2013)
PMID: 23990562

Function and Biology Details

Reaction catalysed: 
UDP-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala) + undecaprenyl phosphate = UMP + Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130312 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phospho-N-acetylmuramoyl-pentapeptide-transferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 365 amino acids
Theoretical weight: 40.95 KDa
Source organism: Aquifex aeolicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O66465 (Residues: 1-359; Coverage: 100%)
Gene names: aq_053, mraY
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand NI 2 x NI
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: P212121
Unit cell:
a: 95.799Å b: 101.491Å c: 138.313Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.227 0.223 0.265
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

19 review citations

Envelope Structures of Gram-Positive Bacteria.
Rajagopal et al. (2017)
18 more

19 mentions without citation

The Membrane Steps of Bacterial Cell Wall Synthesis as Antibiotic Targets.
Liu et al. (2016)
18 more