4j3e Summary


The 1.9A crystal structure of humanized Xenopus Mdm2 with nutlin-3a

The structure was published by Vu, B., Wovkulich, P., Pizzolato, G., et al., Packman, K., Vassilev, L., and Graves, B., in 2013 in a paper entitled "Discovery of RG7112: A Small-Molecule MDM2 Inhibitor in Clinical Development." (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 1.91 Å and deposited in 2013.

The experimental data on which the structure is based was also deposited.

The PDB entry contains the structure of E3 ubiquitin-protein ligase Mdm2. This molecule has the UniProt identifier P56273 (MDM2_XENLA)search. The sample contained 86 residues which is < 90% of the natural sequence. Out of 86 residues 86 were observed and are deposited in the PDB.

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule is most likely monomeric.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):

Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A E3 ubiquitin-protein ligase Mdm2 P56273 (21-105) (MDM2_XENLA)search Xenopus laevissearch < 90% 86 100%

This entry contains 1 unique UniProt protein:

UniProt accession Name Organism PDB
P56273 (21 - 105) E3 ubiquitin-protein ligase Mdm2 Xenopus laevis

Chain Sequence family (Pfam)
A SWIB/MDM2 domainsearch

Chain ID Biological process (GO) Cellular component (GO)
A (P56273) negative regulation of cell cycle arrestsearch negative regulation of apoptotic processsearch protein ubiquitinationsearch negative regulation of transcription from RNA polymerase II promotersearch nucleussearch

Chain InterPro annotation
A SWIB/MDM2 domainsearch Ubiquitin-protein ligase E3 MDM2search