› 4iv0

X-ray diffraction
1.4Å resolution

Crystal structure of N-methyl transferase from Plasmodium vivax complexed with S-adenosyl methionine and phosphate

Released:
DOI: 10.2210/pdb4iv0/pdb
PDB entry 4iv0 coloured by chain, front view.
PDB entry 4iv0 coloured by chain, side view.
PDB entry 4iv0 coloured by chain, top view.
Source organism: Plasmodium vivax Sal-1
Entry authors: Lukk T, Nair SK

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + ethanolamine phosphate = S-adenosyl-L-homocysteine + N-methylethanolamine phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-107689 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
phosphoethanolamine N-methyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 284 amino acids
Theoretical weight: 33.1 KDa
Source organism: Plasmodium vivax Sal-1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A5K867 (Residues: 1-264; Coverage: 100%)
Gene name: PVX_083045
Sequence domains: Methyltransferase domain
Structure domains: Vaccinia Virus protein VP39

Ligands and Environments


Cofactor: Ligand SAM 2 x SAM
3 bound ligands:
Ligand PO4 2 x PO4
Ligand BME 1 x BME
Ligand EDO 1 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P21
Unit cell:
a: 38.17Å b: 86.87Å c: 79.58Å
α: 90° β: 101.05° γ: 90°
R-values:
R R work R free
0.166 0.157 0.178
Expression system: Escherichia coli BL21(DE3)

Quick links