spacer Structure of rat neuronal nitric oxide synthase in complex with 3,5-bis(2-(6-amino-4-methylpyridin-2-yl)ethyl)benzonitrile
Accession P29476search
Keywords Reference proteome, Membrane, Cell projection, Calmodulin-binding, Cell membrane, Flavoprotein, Ubl conjugation, Alternative splicing, Heme, FMN, Complete proteome, Direct protein sequencing, FAD, Iron, Oxidoreductase, Metal-binding, 3D-structure, NADP
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Polymers A B
Enzyme nomenclature
EC number ExPASy BRENDA search
Polymers B, A
Name nitric-oxide synthase, nitric-oxide synthase (NADPH dependent)
Comment The enzyme in brain, but not that induced in lung or liver by endotoxin, requires Ca2+. The stoichiometry is not clear, but may involve a two-electron and a one-electron oxidation step.
Binds FAD, FMN, heme (iron protoporphyrin IX) and tetrahydrobiopterin. This eukaryotic enzyme, which is found in plants and animals [1-3], consists of oxygenase and reductase domains that are linked via a regulatory calmodulin-binding domain. Upon calcium-induced calmodulin binding, the reductase and oxygenase domains form a complex, allowing electrons to flow from NADPH via FAD and FMN to the active center. May produce superoxide under certain conditions. cf. EC, nitric-oxide synthase [NAD(P)H dependent].