4ic1

X-ray diffraction
2.35Å resolution

Function and Biology Details

Reaction catalysed:
Exonucleolytic cleavage in the 5'- to 3'-direction to yield nucleoside 3'-phosphates
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo decamer (preferred)
PDBe Complex ID:
PDB-CPX-188970 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CRISPR-associated exonuclease Cas4 Chains: A, B, C, D, F, G, H, I, J, K
Molecule details ›
Chains: A, B, C, D, F, G, H, I, J, K
Length: 206 amino acids
Theoretical weight: 23.91 KDa
Source organism: Saccharolobus solfataricus P2
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q97TX9 (Residues: 1-202; Coverage: 100%)
Gene names: SSO0001, cas4
Sequence domains:
Structure domains: Lambda Exonuclease; Chain A

Ligands and Environments

2 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID, APS BEAMLINE 19-BM
Spacegroup: P21
Unit cell:
a: 86.152Å b: 197.761Å c: 89.41Å
α: 90° β: 100.88° γ: 90°
R-values:
R R work R free
0.22 0.201 0.249
Expression system: Escherichia coli BL21(DE3)