› 4ibu

X-ray diffraction
1.7Å resolution

Human p53 core domain with hot spot mutation R273C and second-site suppressor mutation T284R in sequence-specific complex with DNA

Released:
DOI: 10.2210/pdb4ibu/pdb
PDB entry 4ibu coloured by chain, front view.
PDB entry 4ibu coloured by chain, side view.
PDB entry 4ibu coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural studies of p53 inactivation by DNA-contact mutations and its rescue by suppressor mutations via alternative protein-DNA interactions.
Eldar A, Rozenberg H, Diskin-Posner Y, Rohs R, Shakked Z
Nucleic Acids Res 41 8748-59 (2013)
PMID: 23863845

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-117637 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Cellular tumor antigen p53 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 200 amino acids
Theoretical weight: 22.51 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P04637 (Residues: 94-293; Coverage: 51%)
Gene names: P53, TP53
Sequence domains: P53 DNA-binding domain
Structure domains: Immunoglobulin-like
DNA (5'-D(*CP*GP*GP*GP*CP*AP*TP*GP*CP*CP*CP*G)-3') Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 12 nucleotides
Theoretical weight: 3.66 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

3 bound ligands:
Ligand ZN 4 x ZN
Ligand EDO 16 x EDO
Ligand ACT 2 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P1
Unit cell:
a: 54.537Å b: 58.158Å c: 77.968Å
α: 82.96° β: 87.78° γ: 73.57°
R-values:
R R work R free
0.164 0.163 0.197
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

9 review citations

The p53 Pathway: Origins, Inactivation in Cancer, and Emerging Therapeutic Approaches.
Joerger et al. (2016)
8 more

5 mentions without citation

Good Cop, Bad Cop: Defining the Roles of Δ40p53 in Cancer and Aging.
Steffens Reinhardt et al. (2020)
4 more