› 4ibp

X-ray diffraction
1.8Å resolution

Crystal structure of a glutathione transferase family member from Pseudomonas fluorescens Pf-5, target EFI-900011, with bound glutathione

Released:
DOI: 10.2210/pdb4ibp/pdb
PDB entry 4ibp coloured by chain, front view.
PDB entry 4ibp coloured by chain, side view.
PDB entry 4ibp coloured by chain, top view.
Source organism: Pseudomonas fluorescens PF5
Entry authors: Vetting MW, Sauder JM, Morisco LL, Wasserman SR, Sojitra S, Imker HJ, Burley SK, Gerlt JA, Almo SC, Enzyme Function Initiative (EFI)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175597 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GST N-terminal domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 214 amino acids
Theoretical weight: 24.41 KDa
Source organism: Pseudomonas fluorescens PF5
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q4K4R5 (Residues: 7-209; Coverage: 97%)
Gene names: PFL_5710, yibF
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand SO4 1 x SO4
Ligand GSH 1 x GSH
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: I212121
Unit cell:
a: 70.531Å b: 81.915Å c: 111.969Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.221 0.22 0.249
Expression system: Escherichia coli BL21(DE3)

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