› 4i49

X-ray diffraction
2.75Å resolution

Structure of ngNAGS bound with bisubstrate analog CoA-NAG

Released:
DOI: 10.2210/pdb4i49/pdb
PDB entry 4i49 coloured by chain, front view.
PDB entry 4i49 coloured by chain, side view.
PDB entry 4i49 coloured by chain, top view.
Source organism: Neisseria gonorrhoeae SK-93-1035
Primary publication:
Structure of the complex of Neisseria gonorrhoeae N-acetyl-L-glutamate synthase with a bound bisubstrate analog.
Zhao G, Allewell NM, Tuchman M, Shi D
Biochem Biophys Res Commun 430 1253-8 (2013)
PMID: 23261468

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-164569 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Amino-acid acetyltransferase Chain: A
Molecule details ›
Chain: A
Length: 456 amino acids
Theoretical weight: 49.24 KDa
Source organism: Neisseria gonorrhoeae SK-93-1035
Expression system: Escherichia coli BL21(DE3)
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand 1C4 1 x 1C4
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: Cu FINE FOCUS
Spacegroup: P312
Unit cell:
a: 98.521Å b: 98.521Å c: 90.009Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.198 0.189 0.271
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 citations in other articles

Structure of N-acetyl-L-glutamate synthase/kinase from Maricaulis maris with the allosteric inhibitor L-arginine bound.
Zhao et al. (2013)

2 mentions without citation

The N-Acetylglutamate Synthase Family: Structures, Function and Mechanisms.
Shi et al. (2015)
1 more