› 4i22

X-ray diffraction
1.71Å resolution

Structure of the monomeric (V948R)gefitinib/erlotinib resistant double mutant (L858R+T790M) EGFR kinase domain co-crystallized with gefitinib

Released:
DOI: 10.2210/pdb4i22/pdb
PDB entry 4i22 coloured by chain, front view.
PDB entry 4i22 coloured by chain, side view.
PDB entry 4i22 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Insights into the aberrant activity of mutant EGFR kinase domain and drug recognition.
Gajiwala KS, Feng J, Ferre R, Ryan K, Brodsky O, Weinrich S, Kath JC, Stewart A
Structure 21 209-19 (2013)
PMID: 23273428

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-132671 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Epidermal growth factor receptor Chain: A
Molecule details ›
Chain: A
Length: 329 amino acids
Theoretical weight: 37.58 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P00533 (Residues: 695-1022; Coverage: 28%)
Gene names: EGFR, ERBB, ERBB1, HER1
Sequence domains: Protein tyrosine and serine/threonine kinase
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand IRE 1 x IRE
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P2
Unit cell:
a: 76.519Å b: 35.486Å c: 77.781Å
α: 90° β: 112.89° γ: 90°
R-values:
R R work R free
0.198 0.197 0.224
Expression system: Spodoptera frugiperda

Quick links

Citations

16 review citations

FDA-approved small-molecule kinase inhibitors.
Wu et al. (2015)
15 more

21 mentions without citation

Globally Approved EGFR Inhibitors: Insights into Their Syntheses, Target Kinases, Biological Activities, Receptor Interactions, and Metabolism.
Abourehab et al. (2021)
20 more