4hwr Summary


Crystal structure of E. coli Threonyl-tRNA synthetase bound to a novel inhibitor

The structure was published by Teng, M., Hilgers, M.T., Cunningham, M.L., et al., Hough, G.W., Shaw, K.J., and Finn, J., in 2013 in a paper entitled "Identification of bacteria-selective threonyl-tRNA synthetase substrate inhibitors by structure-based design." (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 1.9 Å and deposited in 2012.

The experimental data on which the structure is based was also deposited.

This PDB entry contains multiple copies of the structure of Threonine--tRNA ligase.

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule most likely forms homodimers.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):

Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A Threonine--tRNA ligase P0A8M3 (242-642) (SYT_ECOLI)search Escherichia coli K-12search 100% 411 98%
B Threonine--tRNA ligase P0A8M3 (242-642) (SYT_ECOLI)search Escherichia coli K-12search 100% 411 98%

This entry contains 1 unique UniProt protein:

UniProt accession Name Organism PDB
P0A8M3 (242 - 642) Threonine--tRNA ligase Escherichia coli K-12

Chain Sequence family (Pfam)
A, B (P0A8M3) PF00587: tRNA synthetase class II core domain (G, H, P, S and T)search, PF03129: Anticodon binding domainsearch

Chain ID Cellular component (GO) Molecular function (GO) Biological process (GO)
A, B (P0A8M3) cytoplasmsearch nucleotide bindingsearch aminoacyl-tRNA ligase activitysearch threonine-tRNA ligase activitysearch ATP bindingsearch threonyl-tRNA aminoacylationsearch tRNA aminoacylation for protein translationsearch

Chain InterPro annotation
A, B Aminoacyl-tRNA synthetase, class II (G/ H/ P/ S), conserved domainsearch Threonine-tRNA ligase, class IIasearch Anticodon-bindingsearch Aminoacyl-tRNA synthetase, class IIsearch