› 4hwl

X-ray diffraction
2Å resolution

Crystal Structure Analysis of the Bacteriorhodopsin in Facial Amphiphile-7 DMPC Bicelle

Released:
DOI: 10.2210/pdb4hwl/pdb
PDB entry 4hwl coloured by chain, front view.
PDB entry 4hwl coloured by chain, side view.
PDB entry 4hwl coloured by chain, top view.
Source organism: Halobacterium salinarum
Primary publication:
Steroid-based facial amphiphiles for stabilization and crystallization of membrane proteins.
Lee SC, Bennett BC, Hong WX, Fu Y, Baker KA, Marcoux J, Robinson CV, Ward AB, Halpert JR, Stevens RC, Stout CD, Yeager MJ, Zhang Q
Proc Natl Acad Sci U S A 110 E1203-11 (2013)
PMID: 23479627

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136412 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bacteriorhodopsin Chains: A, B
Molecule details ›
Chains: A, B
Length: 262 amino acids
Theoretical weight: 28.27 KDa
Source organism: Halobacterium salinarum
UniProt:
  • Canonical: P02945 (Residues: 1-262; Coverage: 100%)
Gene names: VNG_1467G, bop
Sequence domains: Bacteriorhodopsin-like protein
Structure domains: Rhodopsin 7-helix transmembrane proteins

Ligands and Environments

4 bound ligands:
Ligand RET 2 x RET
Ligand HEX 4 x HEX
Ligand HP6 3 x HP6
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P21
Unit cell:
a: 46.16Å b: 113.29Å c: 56.19Å
α: 90° β: 114.27° γ: 90°
R-values:
R R work R free
0.159 0.159 0.172

Quick links

Citations

11 review citations

Methodological advances: the unsung heroes of the GPCR structural revolution.
Ghosh et al. (2015)
10 more

1 mention without citation

Steroid-based facial amphiphiles for stabilization and crystallization of membrane proteins.
Lee et al. (2013)