› 4huj

X-ray diffraction
1.77Å resolution

Crystal structure of a hypothetical protein SMa0349 from Sinorhizobium meliloti

Released:
DOI: 10.2210/pdb4huj/pdb
PDB entry 4huj coloured by chain, front view.
PDB entry 4huj coloured by chain, side view.
PDB entry 4huj coloured by chain, top view.
Source organism: Sinorhizobium meliloti 1021
Entry authors: Rice S, Eswaramoorthy S, Chamala S, Evans B, Foti F, Gizzi A, Hillerich B, Kar A, LaFleur J, Seidel R, Villigas G, Zencheck W, Almo SC, Swaminathan S, New York Structural Genomics Research Consortium (NYSGRC)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-187990 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pyrroline-5-carboxylate reductase catalytic N-terminal domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 220 amino acids
Theoretical weight: 22.99 KDa
Source organism: Sinorhizobium meliloti 1021
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q930K7 (Residues: 1-198; Coverage: 100%)
Gene name: SMa0349
Sequence domains: NADP oxidoreductase coenzyme F420-dependent
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P212121
Unit cell:
a: 58.456Å b: 79.487Å c: 100.127Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.223 0.206 0.234
Expression system: Escherichia coli BL21

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