› 4hta

X-ray diffraction
1.9Å resolution

The structure of the karrikin insensitive (KAI2) protein in Arabidopsis thaliana

Released:
DOI: 10.2210/pdb4hta/pdb
PDB entry 4hta coloured by chain, front view.
PDB entry 4hta coloured by chain, side view.
PDB entry 4hta coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
The structure of the karrikin-insensitive protein (KAI2) in Arabidopsis thaliana.
Bythell-Douglas R, Waters MT, Scaffidi A, Flematti GR, Smith SM, Bond CS
PLoS One 8 e54758 (2013)
PMID: 23349965

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193566 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Probable esterase KAI2 Chain: A
Molecule details ›
Chain: A
Length: 288 amino acids
Theoretical weight: 31.97 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9SZU7 (Residues: 1-270; Coverage: 100%)
Gene names: At4g37470, D14L, F6G17.120, HTL, KAI2
Sequence domains: Alpha/beta hydrolase family
Structure domains: alpha/beta hydrolase

Ligands and Environments

2 bound ligands:
Ligand GOL 5 x GOL
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P212121
Unit cell:
a: 63.385Å b: 66.064Å c: 77.62Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.156 0.155 0.174
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Strigolactone Signaling and Evolution.
Waters et al. (2017)
7 more

2 mentions without citation

The structure of the karrikin-insensitive protein (KAI2) in Arabidopsis thaliana.
Bythell-Douglas et al. (2013)
1 more