› 4hsn

X-ray diffraction
2Å resolution

Crystal structure of DAH7PS from Neisseria meningitidis

Released:
DOI: 10.2210/pdb4hsn/pdb
PDB entry 4hsn coloured by chain, front view.
PDB entry 4hsn coloured by chain, side view.
PDB entry 4hsn coloured by chain, top view.
Source organism: Neisseria meningitidis MC58
Primary publication:
Neisseria meningitidis expresses a single 3-deoxy-d-arabino-heptulosonate 7-phosphate synthase that is inhibited primarily by phenylalanine.
Cross PJ, Pietersma AL, Allison TM, Wilson-Coutts SM, Cochrane FC, Parker EJ
Protein Sci 22 1087-99 (2013)
PMID: 23754471

Function and Biology Details

Reaction catalysed: 
Phosphoenolpyruvate + D-erythrose 4-phosphate + H(2)O = 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191737 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phospho-2-dehydro-3-deoxyheptonate aldolase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 351 amino acids
Theoretical weight: 38.71 KDa
Source organism: Neisseria meningitidis MC58
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9K169 (Residues: 1-351; Coverage: 100%)
Gene names: NMB0307, aroG
Sequence domains: DAHP synthetase I family
Structure domains: Aldolase class I

Ligands and Environments

3 bound ligands:
Ligand MN 4 x MN
Ligand SO4 4 x SO4
Ligand PEP 4 x PEP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P21
Unit cell:
a: 73.462Å b: 137.279Å c: 76.36Å
α: 90° β: 96.42° γ: 90°
R-values:
R R work R free
0.177 0.175 0.208
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

X-ray Scattering Studies of Protein Structural Dynamics.
Meisburger et al. (2017)
1 more

2 mentions without citation

Neisseria meningitidis expresses a single 3-deoxy-d-arabino-heptulosonate 7-phosphate synthase that is inhibited primarily by phenylalanine.
Cross et al. (2013)
1 more