› 4hre

X-ray diffraction
2.79Å resolution

Crystal Structure of p11/Annexin A2 Heterotetramer in Complex with SMARCA3 Peptide

Released:
DOI: 10.2210/pdb4hre/pdb
PDB entry 4hre coloured by chain, front view.
PDB entry 4hre coloured by chain, side view.
PDB entry 4hre coloured by chain, top view.
Source organisms:
Primary publication:
SMARCA3, a chromatin-remodeling factor, is required for p11-dependent antidepressant action.
Oh YS, Gao P, Lee KW, Ceglia I, Seo JS, Zhang X, Ahn JH, Chait BT, Patel DJ, Kim Y, Greengard P
Cell 152 831-43 (2013)
PMID: 23415230

Function and Biology Details

Reaction catalysed: 
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-139556 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Annexin A2 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 339 amino acids
Theoretical weight: 38.73 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P07356 (Residues: 1-339; Coverage: 100%)
Gene names: Anx2, Anxa2, Cal1h
Sequence domains: Annexin
Structure domains: Annexin
Protein S100-A10 Chains: E, F, I, J
Molecule details ›
Chains: E, F, I, J
Length: 97 amino acids
Theoretical weight: 11.18 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P60903 (Residues: 2-97; Coverage: 99%)
Gene names: ANX2LG, CAL1L, CLP11, S100A10
Sequence domains: S-100/ICaBP type calcium binding domain
Structure domains: EF-hand
Helicase-like transcription factor Chains: G, H, K, L
Molecule details ›
Chains: G, H, K, L
Length: 14 amino acids
Theoretical weight: 1.81 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q14527 (Residues: 26-39; Coverage: 1%)
Gene names: HIP116A, HLTF, RNF80, SMARCA3, SNF2L3, ZBU1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P1
Unit cell:
a: 50.791Å b: 74.346Å c: 154.466Å
α: 90.14° β: 96.56° γ: 108.37°
R-values:
R R work R free
0.257 0.249 0.296
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

18 review citations

The enigmatic mossy cell of the dentate gyrus.
Scharfman HE. (2016)
17 more

7 mentions without citation

Computational Approaches to Toll-Like Receptor 4 Modulation.
Billod et al. (2016)
6 more