› 4hmq

X-ray diffraction
2.1Å resolution

Crystal structure of streptococcus pneumoniae TIGR4 PiaA in complex with ferrichrome

Released:
DOI: 10.2210/pdb4hmq/pdb
PDB entry 4hmq coloured by chain, front view.
PDB entry 4hmq coloured by chain, side view.
PDB entry 4hmq coloured by chain, top view.
Source organism: Streptococcus pneumoniae TIGR4
Primary publication:
Structures of Streptococcus pneumoniae PiaA and its complex with ferrichrome reveal insights into the substrate binding and release of high affinity iron transporters.
Cheng W, Li Q, Jiang YL, Zhou CZ, Chen Y
PLoS One 8 e71451 (2013)
PMID: 23951167

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101436 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fe/B12 periplasmic-binding domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 328 amino acids
Theoretical weight: 36.28 KDa
Source organism: Streptococcus pneumoniae TIGR4
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0H2UPT5 (Residues: 23-341; Coverage: 100%)
Gene name: SP_1032
Sequence domains: Periplasmic binding protein
Structure domains: Nitrogenase molybdenum iron protein domain

Ligands and Environments

5 bound ligands:
Ligand FCE 2 x FCE
Ligand CD 24 x CD
Ligand CL 3 x CL
Ligand EDO 5 x EDO
Ligand PGE 2 x PGE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P212121
Unit cell:
a: 76.102Å b: 84.272Å c: 93.776Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.2 0.248
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Streptococcus pneumoniae's Virulence and Host Immunity: Aging, Diagnostics, and Prevention.
Brooks et al. (2018)
6 more

2 mentions without citation

Structures of Streptococcus pneumoniae PiaA and its complex with ferrichrome reveal insights into the substrate binding and release of high affinity iron transporters.
Cheng et al. (2013)
1 more