› 4hfv

X-ray diffraction
1.9Å resolution

Crystal structure of lpg1851 protein from Legionella pneumophila (putative T4SS effector)

Released:
DOI: 10.2210/pdb4hfv/pdb
PDB entry 4hfv coloured by chain, front view.
PDB entry 4hfv coloured by chain, side view.
PDB entry 4hfv coloured by chain, top view.
Source organism: Legionella pneumophila subsp. pneumophila str. Philadelphia 1
Primary publication:
Diverse mechanisms of metaeffector activity in an intracellular bacterial pathogen, Legionella pneumophila.
Urbanus ML, Quaile AT, Stogios PJ, Morar M, Rao C, Di Leo R, Evdokimova E, Lam M, Oatway C, Cuff ME, Osipiuk J, Michalska K, Nocek BP, Taipale M, Savchenko A, Ensminger AW
Mol Syst Biol 12 893 (2016)
PMID: 27986836

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178646 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dot/Icm T4SS effector Chain: A
Molecule details ›
Chain: A
Length: 190 amino acids
Theoretical weight: 22.21 KDa
Source organism: Legionella pneumophila subsp. pneumophila str. Philadelphia 1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5ZUE7 (Residues: 1-189; Coverage: 86%)
Gene name: lpg1851
Sequence domains: Substrate of the Dot/Icm secretion system, putative
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand CIT 1 x CIT
Ligand SIN 1 x SIN
1 modified residue:
Ligand MSE 2 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P3221
Unit cell:
a: 43.339Å b: 43.339Å c: 205.621Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.185 0.183 0.234
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

19 review citations

Legionella and Coxiella effectors: strength in diversity and activity.
Qiu et al. (2017)
18 more

2 mentions without citation

Diverse mechanisms of metaeffector activity in an intracellular bacterial pathogen, Legionella pneumophila.
Urbanus et al. (2016)
1 more