› 4hbh

X-ray diffraction
2.93Å resolution

Bacterial Photosynthetic Reaction Center from Rhodobacter sphaeroides with ILE M265 replaced with ASN

Released:
DOI: 10.2210/pdb4hbh/pdb
PDB entry 4hbh coloured by chain, front view.
PDB entry 4hbh coloured by chain, side view.
PDB entry 4hbh coloured by chain, top view.
Source organism: Cereibacter sphaeroides
Entry authors: Mattis AJ, Wraight CA

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142934 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Reaction center protein L chain Chain: L
Molecule details ›
Chain: L
Length: 281 amino acids
Theoretical weight: 31.35 KDa
Source organism: Cereibacter sphaeroides
Expression system: Cereibacter sphaeroides
UniProt:
  • Canonical: P0C0Y8 (Residues: 2-282; Coverage: 100%)
Gene name: pufL
Sequence domains: Photosynthetic reaction centre protein
Structure domains: Photosystem II protein D1-like
Reaction center protein M chain Chain: M
Molecule details ›
Chain: M
Length: 313 amino acids
Theoretical weight: 35.23 KDa
Source organism: Cereibacter sphaeroides
Expression system: Cereibacter sphaeroides
UniProt:
  • Canonical: P0C0Y9 (Residues: 2-303; Coverage: 98%)
Gene name: pufM
Sequence domains: Photosynthetic reaction centre protein
Structure domains: Photosystem II protein D1-like
Reaction center protein H chain Chain: H
Molecule details ›
Chain: H
Length: 260 amino acids
Theoretical weight: 28.07 KDa
Source organism: Cereibacter sphaeroides
UniProt:
  • Canonical: P0C0Y7 (Residues: 11-250; Coverage: 92%)
Gene name: puhA
Sequence domains:
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand BCL 4 x BCL
Ligand BPH 2 x BPH
Ligand U10 3 x U10
Ligand FE 1 x FE
Ligand SPO 1 x SPO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P3121
Unit cell:
a: 139.72Å b: 139.72Å c: 183.98Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.187 0.185 0.226
Expression system: Cereibacter sphaeroides

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