› 4h8u

X-ray diffraction
2Å resolution

MUTB inactive double mutant D200A-D415N soaked with sucrose and having as bound ligands sucrose in molecule A and the reaction product trehalulose in molecule B

Released:
DOI: 10.2210/pdb4h8u/pdb
PDB entry 4h8u coloured by chain, front view.
PDB entry 4h8u coloured by chain, side view.
PDB entry 4h8u coloured by chain, top view.
Source organism: Rhizobium sp. MX-45
Entry authors: Lipski A, Haser R, Aghajari N

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125905 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Glycosyl hydrolase family 13 catalytic domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 557 amino acids
Theoretical weight: 63.89 KDa
Source organism: Rhizobium sp. MX-45
Expression system: Escherichia coli
UniProt:
  • Canonical: M1E1F6 (Residues: 28-584; Coverage: 99%)
Gene name: mutB
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC, FRU
Carbohydrate polymer
3 bound ligands:
Ligand GOL 1 x GOL
Ligand CA 2 x CA
Ligand TEU 1 x TEU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P1
Unit cell:
a: 64.37Å b: 73.68Å c: 82.67Å
α: 66.15° β: 74.29° γ: 72.42°
R-values:
R R work R free
0.192 0.189 0.257
Expression system: Escherichia coli

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