4h75

X-ray diffraction
2.1Å resolution

Crystal structure of human Spindlin1 in complex with a histone H3K4(me3) peptide

Released:
Source organism: Homo sapiens

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-178333 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Spindlin-1 Chain: A
Molecule details ›
Chain: A
Length: 238 amino acids
Theoretical weight: 27.13 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9Y657 (Residues: 27-262; Coverage: 90%)
Gene names: OCR, SPIN, SPIN1
Sequence domains: Spin/Ssty Family
Structure domains: Spindlin/Ssty
Histone H3-7 Chain: B
Molecule details ›
Chain: B
Length: 8 amino acids
Theoretical weight: 976 Da
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q5TEC6 (Residues: 2-9; Coverage: 6%)
Gene names: H3-2, H3-7, HIST2H3PS2

Ligands and Environments

3 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P21212
Unit cell:
a: 122.429Å b: 42.685Å c: 50.307Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.183 0.181 0.218
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided