› 4grp

X-ray diffraction
1.27Å resolution

Crystallographic and biological characterization of N- and C- terminus mutants of human MIF

Released:
DOI: 10.2210/pdb4grp/pdb
PDB entry 4grp coloured by chain, front view.
PDB entry 4grp coloured by chain, side view.
PDB entry 4grp coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Fan C, Lolis E

Function and Biology Details

Reactions catalysed: 
Keto-phenylpyruvate = enol-phenylpyruvate
L-dopachrome = 5,6-dihydroxyindole-2-carboxylate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146863 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Macrophage migration inhibitory factor Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 118 amino acids
Theoretical weight: 12.64 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P14174 (Residues: 3-115; Coverage: 98%)
Gene names: GLIF, MIF, MMIF
Sequence domains: Macrophage migration inhibitory factor (MIF)
Structure domains: Macrophage Migration Inhibitory Factor

Ligands and Environments

2 bound ligands:
Ligand SO4 5 x SO4
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Unit cell:
a: 50.05Å b: 76.25Å c: 82.05Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.138 0.137 0.169
Expression system: Escherichia coli BL21(DE3)

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