› 4gpk

X-ray diffraction
3.2Å resolution

Crystal structure of NprR in complex with its cognate peptide NprX

Released:
DOI: 10.2210/pdb4gpk/pdb
PDB entry 4gpk coloured by chain, front view.
PDB entry 4gpk coloured by chain, side view.
PDB entry 4gpk coloured by chain, top view.
Source organism: Bacillus thuringiensis serovar thuringiensis
Primary publication:
Peptide-binding dependent conformational changes regulate the transcriptional activity of the quorum-sensor NprR.
Zouhir S, Perchat S, Nicaise M, Perez J, Guimaraes B, Lereclus D, Nessler S
Nucleic Acids Res 41 7920-33 (2013)
PMID: 23793817

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124482 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
NprR Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 372 amino acids
Theoretical weight: 44.53 KDa
Source organism: Bacillus thuringiensis serovar thuringiensis
Expression system: Escherichia coli
UniProt:
  • Canonical: G5DDY8 (Residues: 11-374; Coverage: 97%)
Gene name: nprR
Sequence domains: Tetratricopeptide repeat
Structure domains:
NprX peptide Chains: M, N, O, P, Q, R, S, T, U, V, W, X
Molecule details ›
Chains: M, N, O, P, Q, R, S, T, U, V, W, X
Length: 8 amino acids
Theoretical weight: 803 Da
Source organism: Bacillus thuringiensis serovar thuringiensis
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P1
Unit cell:
a: 122.24Å b: 133.35Å c: 137.5Å
α: 108.25° β: 104.83° γ: 103.83°
R-values:
R R work R free
0.276 0.27 0.299
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

9 review citations

The Bacillus cereus Group: Bacillus Species with Pathogenic Potential.
Ehling-Schulz et al. (2019)
8 more

8 mentions without citation

Structural Mechanisms of Peptide Recognition and Allosteric Modulation of Gene Regulation by the RRNPP Family of Quorum-Sensing Regulators.
Do et al. (2016)
7 more