4gnk Summary

pdbe.org/4gnk
spacer

Crystal structure of Galphaq in complex with full-length human PLCbeta3

The structure was published by Lyon, A.M., Dutta, S., Boguth, C.A., Skiniotis, G., and Tesmer, J.J., in 2013 in a paper entitled "Full-length G alpha (q)-phospholipase C-beta 3 structure reveals interfaces of the C-terminal coiled-coil domain." (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 4.0 Å and deposited in 2012.

The experimental data on which the structure is based was also deposited.

This PDB entry contains a complex of 3 biomacromolecules, namely Guanine nucleotide-binding protein G(q) subunit alpha and 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3.

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule has more than one probable quaternary state observed. For more details see the quaternary structure page.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):


Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A Guanine nucleotide-binding protein G(q) subunit alpha P21279 (7-359) (GNAQ_MOUSE)search Mus musculussearch 90% 353 95%
C Guanine nucleotide-binding protein G(q) subunit alpha P21279 (7-359) (GNAQ_MOUSE)search Mus musculussearch 90% 353 95%
B 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3 Q01970 (10-1234) (PLCB3_HUMAN)search Homo sapienssearch 99% 1235 62%
D 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3 Q01970 (10-1234) (PLCB3_HUMAN)search Homo sapienssearch 99% 1235 62%
E 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3 Q01970 (934-1192) (PLCB3_HUMAN)search Homo sapienssearch 99% 259 90%


This entry contains 2 unique UniProt proteins:

UniProt accession Name Organism PDB
P21279 (7 - 359) Guanine nucleotide-binding protein G(q) subunit alpha Mus musculus
Q01970 (10 - 1234) 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3 Homo sapiens

Chain Sequence family (Pfam)
A, C (P21279) PF00503: G-protein alpha subunitsearch
B, D (Q01970) PF00168: C2 domainsearch, PF00387: Phosphatidylinositol-specific phospholipase C, Y domainsearch, PF00388: Phosphatidylinositol-specific phospholipase C, X domainsearch, PF06631: Protein of unknown function (DUF1154)search, PF08703: PLC-beta C terminalsearch, PF09279: Phosphoinositide-specific phospholipase C, efhand-likesearch
E (Q01970) PF06631: Protein of unknown function (DUF1154)search, PF08703: PLC-beta C terminalsearch

Chain ID Molecular function (GO) Biological process (GO) Cellular component (GO)
A, C (P21279) metal ion bindingsearch G-protein beta/gamma-subunit complex bindingsearch GTPase activator activitysearch protein bindingsearch signal transducer activitysearch G-protein coupled receptor bindingsearch guanyl nucleotide bindingsearch GTPase activitysearch GTP bindingsearch nucleotide bindingsearch positive regulation of GTPase activitysearch regulation of catenin import into nucleussearch G-protein coupled receptor signaling pathwaysearch developmental pigmentationsearch neuron remodelingsearch GTP catabolic processsearch signal transductionsearch protein stabilizationsearch regulation of melanocyte differentiationsearch maternal behaviorsearch glutamate receptor signaling pathwaysearch phospholipase C-activating dopamine receptor signaling pathwaysearch adenylate cyclase-activating G-protein coupled receptor signaling pathwaysearch forebrain neuron developmentsearch regulation of action potentialsearch post-embryonic developmentsearch negative regulation of protein kinase activitysearch embryonic digit morphogenesissearch heart developmentsearch skeletal system developmentsearch heterotrimeric G-protein complexsearch membranesearch nucleussearch plasma membranesearch extrinsic to internal side of plasma membranesearch nuclear membranesearch
B, D (Q01970) signal transducer activitysearch phosphatidylinositol phospholipase C activitysearch calcium ion bindingsearch phosphoric diester hydrolase activitysearch phospholipase C activitysearch calmodulin bindingsearch hydrolase activitysearch regulation of systemic arterial blood pressuresearch lipid catabolic processsearch signal transductionsearch intracellular signal transductionsearch lipid metabolic processsearch inositol phosphate metabolic processsearch synaptic transmissionsearch small molecule metabolic processsearch cytosolsearch membranesearch protein complexsearch
E (Q01970) phosphatidylinositol phospholipase C activitysearch calcium ion bindingsearch lipid metabolic processsearch intracellular signal transductionsearch lipid catabolic processsearch

Chain InterPro annotation
A, C G-protein alpha subunit, group Qsearch Guanine nucleotide binding protein (G-protein), alpha subunitsearch G protein alpha subunit, helical insertionsearch P-loop containing nucleoside triphosphate hydrolasesearch
B, D C2 domainsearch Phospholipase C, phosphatidylinositol-specific , X domainsearch Phosphoinositide phospholipase C familysearch Phospholipase C, phosphatidylinositol-specific, Y domainsearch search Phosphatidylinositol-4, 5-bisphosphate phosphodiesterase beta, conserved sitesearch EF-hand domain pairsearch Pleckstrin homology-like domainsearch PLC-beta, C-terminalsearch Phospholipase C, phosphoinositol-specific, EF-hand-likesearch Phosphatidylinositol-4, 5-bisphosphate phosphodiesterase betasearch PLC-like phosphodiesterase, TIM beta/alpha-barrel domainsearch search
E Phosphoinositide phospholipase C familysearch Phosphatidylinositol-4, 5-bisphosphate phosphodiesterase beta, conserved sitesearch PLC-beta, C-terminalsearch