› 4gkl

X-ray diffraction
2.4Å resolution

Crystal structure of a noncanonic maltogenic alpha-amylase AmyB from Thermotoga neapolitana

Released:
DOI: 10.2210/pdb4gkl/pdb
PDB entry 4gkl coloured by chain, front view.
PDB entry 4gkl coloured by chain, side view.
PDB entry 4gkl coloured by chain, top view.
Source organism: Thermotoga neapolitana
Primary publication:
Structure of a novel α-amylase AmyB from Thermotoga neapolitana that produces maltose from the nonreducing end of polysaccharides.
Jun SY, Kim JS, Choi KH, Cha J, Ha NC
Acta Crystallogr D Biol Crystallogr 69 442-50 (2013)
PMID: 23519419

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110171 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycosyl hydrolase family 13 catalytic domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 422 amino acids
Theoretical weight: 50.11 KDa
Source organism: Thermotoga neapolitana
Expression system: Escherichia coli
UniProt:
  • Canonical: B5ARZ9 (Residues: 1-422; Coverage: 100%)
Gene name: amyB
Sequence domains: Alpha amylase, catalytic domain
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NE3A
Spacegroup: P21
Unit cell:
a: 66.88Å b: 74.449Å c: 108.9Å
α: 90° β: 107.77° γ: 90°
R-values:
R R work R free
0.201 0.197 0.263
Expression system: Escherichia coli

Quick links

Citations

2 citation in other articles

Characterization and gene cloning of a maltotriose-forming exo-amylase from Kitasatospora sp. MK-1785.
Kamon et al. (2015)
1 more