› 4gha

X-ray diffraction
2.5Å resolution

Crystal structure of Marburg virus VP35 RNA binding domain bound to 12-bp dsRNA

Released:
DOI: 10.2210/pdb4gha/pdb
PDB entry 4gha coloured by chain, front view.
PDB entry 4gha coloured by chain, side view.
PDB entry 4gha coloured by chain, top view.
Source organism: Marburg virus - Musoke, Kenya, 1980
Primary publication:
Marburg virus VP35 can both fully coat the backbone and cap the ends of dsRNA for interferon antagonism.
Bale S, Julien JP, Bornholdt ZA, Kimberlin CR, Halfmann P, Zandonatti MA, Kunert J, Kroon GJ, Kawaoka Y, MacRae IJ, Wilson IA, Saphire EO
PLoS Pathog 8 e1002916 (2012)
PMID: 23028316

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152875 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Polymerase cofactor VP35 Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 146 amino acids
Theoretical weight: 16.3 KDa
Source organism: Marburg virus - Musoke, Kenya, 1980
Expression system: Escherichia coli
UniProt:
  • Canonical: P35259 (Residues: 204-329; Coverage: 38%)
Gene name: VP35
Sequence domains: Filoviridae VP35
Structure domains:
RNA (5'-R(*CP*UP*AP*GP*AP*CP*GP*UP*CP*UP*AP*G)-3') Chains: I, J
Molecule details ›
Chains: I, J
Length: 12 nucleotides
Theoretical weight: 3.81 KDa
Source organism: Marburg virus - Musoke, Kenya, 1980
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P21
Unit cell:
a: 36.593Å b: 99.191Å c: 81.801Å
α: 90° β: 89.92° γ: 90°
R-values:
R R work R free
0.211 0.209 0.25
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

20 review citations

Filovirus pathogenesis and immune evasion: insights from Ebola virus and Marburg virus.
Messaoudi et al. (2015)
19 more

7 mentions without citation

Strategies of highly pathogenic RNA viruses to block dsRNA detection by RIG-I-like receptors: hide, mask, hit.
Zinzula et al. (2013)
6 more