4gfj

X-ray diffraction
2.91Å resolution

Crystal structure of Topo-78, an N-terminal 78kDa fragment of topoisomerase V

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
monomeric (preferred)
PDBe Complex ID:
PDB-CPX-123367 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Helix-hairpin-helix DNA-binding motif class 1 domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 685 amino acids
Theoretical weight: 78.68 KDa
Source organism: Methanopyrus kandleri AV19
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: F1SVL0 (Residues: 1-685; Coverage: 70%)
Gene name: MK1436
Sequence domains:
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P61
Unit cell:
a: 161.634Å b: 161.634Å c: 58.227Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.205 0.201 0.267
Expression system: Escherichia coli BL21(DE3)