› 4gcv

X-ray diffraction
2.3Å resolution

Structure of a Putative transcription factor (PA1374)from Pseudomonas aeruginosa

Released:
DOI: 10.2210/pdb4gcv/pdb
PDB entry 4gcv coloured by chain, front view.
PDB entry 4gcv coloured by chain, side view.
PDB entry 4gcv coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Primary publication:
The X-ray crystal structure of PA1374 from Pseudomonas aeruginosa, a putative oxidative-stress sensing transcriptional regulator.
Kim H, Choe J
Biochem Biophys Res Commun 431 376-81 (2013)
PMID: 23337505

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191348 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH hxlR-type domain-containing protein Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 168 amino acids
Theoretical weight: 19.52 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9I3X0 (Residues: 1-168; Coverage: 100%)
Gene name: PA1374
Sequence domains: HxlR-like helix-turn-helix
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain

Ligands and Environments

3 bound ligands:
Ligand NA 11 x NA
Ligand GOL 12 x GOL
Ligand PO4 4 x PO4
1 modified residue:
Ligand MSE 84 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 4A
Spacegroup: C2
Unit cell:
a: 128.897Å b: 74.448Å c: 230.492Å
α: 90° β: 90.03° γ: 90°
R-values:
R R work R free
0.218 0.214 0.287
Expression system: Escherichia coli

Quick links

Citations

3 citation in other articles

Structural mechanism of transcription regulation of the Staphylococcus aureus multidrug efflux operon mepRA by the MarR family repressor MepR.
Birukou et al. (2014)
2 more