› 4gck

X-ray diffraction
2.05Å resolution

structure of no-dna complex

Released:
DOI: 10.2210/pdb4gck/pdb
PDB entry 4gck coloured by chain, front view.
PDB entry 4gck coloured by chain, side view.
PDB entry 4gck coloured by chain, top view.
Source organism: Klebsiella pneumoniae 342
Primary publication:
SlmA forms a higher-order structure on DNA that inhibits cytokinetic Z-ring formation over the nucleoid.
Tonthat NK, Milam SL, Chinnam N, Whitfill T, Margolin W, Schumacher MA
Proc Natl Acad Sci U S A 110 10586-91 (2013)
PMID: 23754405

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110261 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Nucleoid occlusion factor SlmA Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 212 amino acids
Theoretical weight: 24.38 KDa
Source organism: Klebsiella pneumoniae 342
Expression system: Escherichia coli
UniProt:
  • Canonical: B5XTG2 (Residues: 1-198; Coverage: 100%)
Gene names: KPK_0113, slmA
Sequence domains: Bacterial regulatory proteins, tetR family
Structure domains: Tetracycline Repressor, domain 2
DNA (5'-D(*GP*TP*GP*AP*GP*TP*AP*CP*TP*CP*AP*C)-3') Chains: W, Z
Molecule details ›
Chains: W, Z
Length: 12 nucleotides
Theoretical weight: 3.66 KDa
Source organism: Klebsiella pneumoniae 342
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P212121
Unit cell:
a: 71.49Å b: 80.75Å c: 201.3Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.218 0.218 0.243
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

17 review citations

Subcellular Organization: A Critical Feature of Bacterial Cell Replication.
Surovtsev et al. (2018)
16 more

3 mentions without citation

SlmA forms a higher-order structure on DNA that inhibits cytokinetic Z-ring formation over the nucleoid.
Tonthat et al. (2013)
2 more