› 4g8t

X-ray diffraction
1.7Å resolution

Crystal structure of a glucarate dehydratase related protein, from actinobacillus succinogenes, target EFI-502312, with sodium and sulfate bound, ordered loop

Released:
DOI: 10.2210/pdb4g8t/pdb
PDB entry 4g8t coloured by chain, front view.
PDB entry 4g8t coloured by chain, side view.
PDB entry 4g8t coloured by chain, top view.
Source organism: Actinobacillus succinogenes 130Z
Entry authors: Vetting MW, Toro R, Bhosle R, Al Obaidi NF, Morisco LL, Wasserman SR, Sojitra S, Washington E, Scott Glenn A, Chowdhury S, Evans B, Hammonds J, Hillerich B, Love J, Seidel RD, Imker HJ, Gerlt JA, Almo SC, Enzyme Function Initiative (EFI)

Function and Biology Details

Reaction catalysed: 
D-glucarate = 5-dehydro-4-deoxy-D-glucarate + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-108139 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
glucarate dehydratase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 464 amino acids
Theoretical weight: 51.46 KDa
Source organism: Actinobacillus succinogenes 130Z
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A6VQF1 (Residues: 1-442; Coverage: 100%)
Gene name: Asuc_1847
Sequence domains: Enolase C-terminal domain-like
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand SO4 4 x SO4
Ligand NA 4 x NA
Ligand GOL 7 x GOL
Ligand DTU 1 x DTU
Ligand DTT 3 x DTT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P212121
Unit cell:
a: 110.837Å b: 124.854Å c: 139.093Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.154 0.152 0.179
Expression system: Escherichia coli BL21(DE3)

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