› 4g4p

X-ray diffraction
1.5Å resolution

Crystal structure of glutamine-binding protein from Enterococcus faecalis at 1.5 A

Released:
DOI: 10.2210/pdb4g4p/pdb
PDB entry 4g4p coloured by chain, front view.
PDB entry 4g4p coloured by chain, side view.
PDB entry 4g4p coloured by chain, top view.
Source organism: Enterococcus faecalis V583
Primary publication:
Functional diversity of tandem substrate-binding domains in ABC transporters from pathogenic bacteria.
Fulyani F, Schuurman-Wolters GK, Zagar AV, Guskov A, Slotboom DJ, Poolman B
Structure 21 1879-88 (2013)
PMID: 23994008

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182849 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ABC transmembrane type-1 domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 244 amino acids
Theoretical weight: 27.3 KDa
Source organism: Enterococcus faecalis V583
Expression system: Escherichia coli
UniProt:
  • Canonical: Q837S0 (Residues: 29-252; Coverage: 32%)
Gene name: EF_0761
Sequence domains: Bacterial extracellular solute-binding proteins, family 3
Structure domains: Periplasmic binding protein-like II

Ligands and Environments

2 bound ligands:
Ligand MES 1 x MES
Ligand GLN 1 x GLN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P21
Unit cell:
a: 40.73Å b: 61.13Å c: 45.12Å
α: 90° β: 99.05° γ: 90°
R-values:
R R work R free
0.117 0.115 0.155
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Diversity in ABC transporters: type I, II and III importers.
Rice et al. (2014)
3 more

1 mention without citation

L-glutamine Induces Expression of Listeria monocytogenes Virulence Genes.
Haber et al. (2017)