› 4g33

X-ray diffraction
2.03Å resolution

Crystal Structure of a Phospholipid-Lipoxygenase Complex from Pseudomonas aeruginosa at 2.0 A (C2221)

Released:
DOI: 10.2210/pdb4g33/pdb
PDB entry 4g33 coloured by chain, front view.
PDB entry 4g33 coloured by chain, side view.
PDB entry 4g33 coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Structure and interaction with phospholipids of a prokaryotic lipoxygenase from Pseudomonas aeruginosa.
Garreta A, Val-Moraes SP, García-Fernández Q, Busquets M, Juan C, Oliver A, Ortiz A, Gaffney BJ, Fita I, Manresa À, Carpena X
FASEB J 27 4811-21 (2013)
PMID: 23985801

Function and Biology Details

Reactions catalysed: 
Alpha-linolenate + O(2) = (9Z,11E,13S,15Z)-13-hydroperoxyoctadeca-9,11,15-trienoate
Linoleate + O(2) = (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate
Alpha-linolenate + O(2) = (8E,10S,12Z,15Z)-10-hydroperoxyoctadeca-8,12,15-trienoate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-186011 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Linoleate 9/13-lipoxygenase Chain: A
Molecule details ›
Chain: A
Length: 688 amino acids
Theoretical weight: 75.05 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8RNT4 (Residues: 19-685; Coverage: 100%)
Gene name: lox
Sequence domains: Lipoxygenase

Ligands and Environments

3 bound ligands:
Ligand FE2 1 x FE2
Ligand ZPE 1 x ZPE
Ligand GOL 5 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: C2221
Unit cell:
a: 84.85Å b: 97.276Å c: 157.16Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.18 0.225
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Mammalian lipoxygenases and their biological relevance.
Kuhn et al. (2015)
9 more

6 mentions without citation

The structural basis for specificity in lipoxygenase catalysis.
Newcomer et al. (2015)
5 more