› 4g1h

X-ray diffraction
1.8Å resolution

Group B Streptococcus Pilus Island 1 Sortase C2

Released:
DOI: 10.2210/pdb4g1h/pdb
PDB entry 4g1h coloured by chain, front view.
PDB entry 4g1h coloured by chain, side view.
PDB entry 4g1h coloured by chain, top view.
Source organism: Streptococcus agalactiae serogroup V
Primary publication:
Structural basis for group B streptococcus pilus 1 sortases C regulation and specificity.
Cozzi R, Prigozhin D, Rosini R, Abate F, Bottomley MJ, Grandi G, Telford JL, Rinaudo CD, Maione D, Alber T
PLoS One 7 e49048 (2012)
PMID: 23145064

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184286 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sortase family protein Chain: A
Molecule details ›
Chain: A
Length: 215 amino acids
Theoretical weight: 24.02 KDa
Source organism: Streptococcus agalactiae serogroup V
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8E0S6 (Residues: 19-233; Coverage: 83%)
Gene name: SAG0648
Sequence domains: Sortase domain
Structure domains: Sortase

Ligands and Environments

1 bound ligand:
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P41212
Unit cell:
a: 60.435Å b: 60.435Å c: 102.242Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.212 0.251
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Sortagging: a robust and efficient chemoenzymatic ligation strategy.
Ritzefeld M. (2014)
4 more

3 mentions without citation

Sortase Transpeptidases: Structural Biology and Catalytic Mechanism.
Jacobitz et al. (2017)
2 more