4g0w Summary


Human topoisomerase iibeta in complex with DNA and ametantrone

The structure was published by Wu, C.C., Li, Y.C., Wang, Y.R., Li, T.K., and Chan, N.L., in 2013 in a paper entitled "On the structural basis and design guidelines for type II topoisomerase-targeting anticancer drugs" (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 2.696 Å and deposited in 2012.

The experimental data on which the structure is based was also deposited.

This PDB entry contains a complex of 3 biomacromolecules, namely DNA topoisomerase 2-beta, DNA (5'-D(P*AP*GP*CP*CP*GP*AP*GP*C)-3'), and DNA (5'-D(P*TP*GP*CP*AP*GP*CP*TP*CP*GP*GP*CP*T)-3').

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule most likely forms heterohexamers.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):

Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A DNA topoisomerase 2-beta Q02880 (450-1206) (TOP2B_HUMAN)search Homo sapienssearch < 90% 803 82%
B DNA topoisomerase 2-beta Q02880 (450-1206) (TOP2B_HUMAN)search Homo sapienssearch < 90% 803 82%

This entry contains 1 unique UniProt protein:

UniProt accession Name Organism PDB
Q02880 (450 - 1206) DNA topoisomerase 2-beta Homo sapiens

Chain Sequence family (Pfam)
A, B DNA gyrase Bsearch, DNA gyrase/topoisomerase IV, subunit Asearch, Toprim domainsearch

Chain ID Molecular function (GO) Biological process (GO)
A, B (Q02880) DNA bindingsearch ATP bindingsearch DNA topoisomerase type II (ATP-hydrolyzing) activitysearch DNA binding, bendingsearch DNA topological changesearch DNA metabolic processsearch

Chain InterPro annotation
A, B DNA topoisomerase II, eukaryotic-typesearch DNA topoisomerase, type IIAsearch DNA topoisomerase, type IIA, subunit A/C-terminalsearch Toprim domainsearch Type IIA DNA topoisomerase subunit A, alpha-helical domainsearch DNA topoisomerase, type IIA, subunit A/ C-terminal, alpha-betasearch DNA topoisomerase, type IIA, central domainsearch DNA topoisomerase, type IIA-like domainsearch DNA topoisomerase, type IIA, conserved sitesearch Arginine repressor C-terminal-like domainsearch DNA topoisomerase II-betasearch