› 4fyo

X-ray diffraction
1.4Å resolution

Crystal structure of spleen tyrosine kinase complexed with N-{(S)-1-[7-(3,4-Dimethoxy-phenylamino)-thiazolo[5,4-d]pyrimidin-5-yl]-pyrrolidin-3-yl}-terephthalamic acid

Released:
DOI: 10.2210/pdb4fyo/pdb
PDB entry 4fyo coloured by chain, front view.
PDB entry 4fyo coloured by chain, side view.
PDB entry 4fyo coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Rational design of highly selective spleen tyrosine kinase inhibitors.
Lucas MC, Goldstein DM, Hermann JC, Kuglstatter A, Liu W, Luk KC, Padilla F, Slade M, Villaseñor AG, Wanner J, Xie W, Zhang X, Liao C
J Med Chem 55 10414-23 (2012)
PMID: 23151054

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155056 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tyrosine-protein kinase SYK Chain: A
Molecule details ›
Chain: A
Length: 291 amino acids
Theoretical weight: 33.88 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P43405 (Residues: 356-635; Coverage: 44%)
Gene name: SYK
Sequence domains: Protein tyrosine and serine/threonine kinase
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand 0VF 1 x 0VF
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P212121
Unit cell:
a: 39.953Å b: 85.563Å c: 90.031Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.219 0.24
Expression system: Spodoptera frugiperda

Quick links

Citations

1 review citation

Small-molecule inhibitors of spleen tyrosine kinase as therapeutic agents for immune disorders: will promise meet expectations?
Lucas et al. (2014)
18 other articles

3 mentions without citation

Best practices for constructing, preparing, and evaluating protein-ligand binding affinity benchmarks [Article v0.1].
Hahn et al. (2022)
2 more