› 4fu0

X-ray diffraction
2.35Å resolution

Crystal Structure of VanG D-Ala:D-Ser Ligase from Enterococcus faecalis

Released:
DOI: 10.2210/pdb4fu0/pdb
PDB entry 4fu0 coloured by chain, front view.
PDB entry 4fu0 coloured by chain, side view.
PDB entry 4fu0 coloured by chain, top view.
Source organism: Enterococcus faecalis
Primary publication:
Structural and functional characterization of VanG D-Ala:D-Ser ligase associated with vancomycin resistance in Enterococcus faecalis.
Meziane-Cherif D, Saul FA, Haouz A, Courvalin P
J Biol Chem 287 37583-92 (2012)
PMID: 22969085

Function and Biology Details

Reaction catalysed: 
ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180502 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
D-alanine--D-alanine ligase Chains: A, B
Molecule details ›
Chains: A, B
Length: 357 amino acids
Theoretical weight: 39.84 KDa
Source organism: Enterococcus faecalis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6WRY5 (Residues: 1-349; Coverage: 100%)
Gene names: ddl, vanG
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ADP 2 x ADP
Ligand SO4 9 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P3121
Unit cell:
a: 116.07Å b: 116.07Å c: 177.228Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.19 0.189 0.224
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Resistance to antibiotics targeted to the bacterial cell wall.
Nikolaidis et al. (2014)
1 more

3 mentions without citation

Molecular mechanisms of vancomycin resistance.
Stogios et al. (2020)
2 more