› 4fn7

X-ray diffraction
1.25Å resolution

Apo Structure of the Mtb enoyol CoA isomerase (Rv0632c)

Released:
DOI: 10.2210/pdb4fn7/pdb
PDB entry 4fn7 coloured by chain, front view.
PDB entry 4fn7 coloured by chain, side view.
PDB entry 4fn7 coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Entry authors: Bruning JB, Gao N, Hernandez ED, Li H, Dang N, Hung LW, Sacchettini JC, TB Structural Genomics Consortium (TBSGC)

Function and Biology Details

Reaction catalysed: 
(3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H(2)O
Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-235702 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Probable enoyl-CoA hydratase EchA3 (Enoyl hydrase) (Unsaturated acyl-CoA hydratase) (Crotonase) Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 231 amino acids
Theoretical weight: 24.38 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: I6Y8B5 (Residues: 1-231; Coverage: 100%)
Gene names: Rv0632c, echA3
Sequence domains: Enoyl-CoA hydratase/isomerase
Structure domains: 2-enoyl-CoA Hydratase; Chain A, domain 1

Ligands and Environments

1 bound ligand:
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P21
Unit cell:
a: 64.132Å b: 68.681Å c: 75.57Å
α: 90° β: 108.57° γ: 90°
R-values:
R R work R free
0.15 0.149 0.183
Expression system: Escherichia coli BL21(DE3)

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