Function and Biology Details
Reaction catalysed:
Endohydrolysis of RNA in RNA/DNA hybrids. Three different cleavage modes: 1. sequence-specific internal cleavage of RNA. Human immunodeficiency virus type 1 and Moloney murine leukemia virus enzymes prefer to cleave the RNA strand one nucleotide away from the RNA-DNA junction. 2. RNA 5'-end directed cleavage 13-19 nucleotides from the RNA end. 3. DNA 3'-end directed cleavage 15-20 nucleotides away from the primer terminus.
Biochemical function:
Biological process:
Cellular component:
- not assigned
Structure analysis Details
Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169396 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Pol protein
Molecule details ›
Chains: A, B
Length: 99 amino acids
Theoretical weight: 10.77 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Escherichia coli
UniProt:
Sequence domains: Retroviral aspartyl protease
Structure domains: Acid Proteases
Length: 99 amino acids
Theoretical weight: 10.77 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Escherichia coli
UniProt:
- Canonical:
Q000H7 (Residues: 1-99; Coverage: 25%)
Sequence domains: Retroviral aspartyl protease
Structure domains: Acid Proteases
Substrate p2/NC peptide
Molecule details ›
Chain: D
Length: 7 amino acids
Theoretical weight: 837 Da
Source organism: Human immunodeficiency virus 1
Expression system: Not provided
UniProt:
Length: 7 amino acids
Theoretical weight: 837 Da
Source organism: Human immunodeficiency virus 1
Expression system: Not provided
UniProt:
- Canonical: Q9YP46 (Residues: 375-381; Coverage: 1%)
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
X-ray source:
APS BEAMLINE 21-ID-D
Spacegroup:
P212121
Expression systems:
- Escherichia coli
- Not provided
