› 4f26

X-ray diffraction
2Å resolution

Crystal structure of the second RRM domain of human PABPC1 a pH 9.0

Released:
DOI: 10.2210/pdb4f26/pdb
PDB entry 4f26 coloured by chain, front view.
PDB entry 4f26 coloured by chain, side view.
PDB entry 4f26 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Interdomain allostery promotes assembly of the poly(A) mRNA complex with PABP and eIF4G.
Safaee N, Kozlov G, Noronha AM, Xie J, Wilds CJ, Gehring K
Mol Cell 48 375-86 (2012)
PMID: 23041282

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145989 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Polyadenylate-binding protein 1 Chain: A
Molecule details ›
Chain: A
Length: 115 amino acids
Theoretical weight: 12.68 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P11940 (Residues: 99-199; Coverage: 16%)
Gene names: PAB1, PABP, PABP1, PABPC1, PABPC2
Sequence domains: RNA recognition motif
Structure domains: Alpha-Beta Plaits

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: C2221
Unit cell:
a: 52.955Å b: 69.487Å c: 41.787Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.219 0.216 0.286
Expression system: Escherichia coli BL21

Quick links

Citations

18 review citations

How RNA-Binding Proteins Interact with RNA: Molecules and Mechanisms.
Corley et al. (2020)
17 more

1 mention without citation

The crystal structure of the Split End protein SHARP adds a new layer of complexity to proteins containing RNA recognition motifs.
Arieti et al. (2014)