› 4ex7

X-ray diffraction
1.5Å resolution

Crystal structure of the alnumycin P phosphatase in complex with free phosphate

Released:
DOI: 10.2210/pdb4ex7/pdb
PDB entry 4ex7 coloured by chain, front view.
PDB entry 4ex7 coloured by chain, side view.
PDB entry 4ex7 coloured by chain, top view.
Source organism: Streptomyces sp. CM020
Primary publication:
Structural basis for C-ribosylation in the alnumycin A biosynthetic pathway.
Oja T, Niiranen L, Sandalova T, Klika KD, Niemi J, Mäntsälä P, Schneider G, Metsä-Ketelä M
Proc Natl Acad Sci U S A 110 1291-6 (2013)
PMID: 23297194

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110379 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
AlnB Chain: A
Molecule details ›
Chain: A
Length: 237 amino acids
Theoretical weight: 24.25 KDa
Source organism: Streptomyces sp. CM020
Expression system: Escherichia coli
UniProt:
  • Canonical: B6SEG4 (Residues: 2-227; Coverage: 100%)
Gene name: alnB
Sequence domains: Haloacid dehalogenase-like hydrolase
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand MG 1 x MG
Ligand BO3 1 x BO3
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P212121
Unit cell:
a: 48.256Å b: 63.228Å c: 63.491Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.146 0.144 0.186
Expression system: Escherichia coli

Quick links

Citations

7 review citations

A comprehensive review of glycosylated bacterial natural products.
Elshahawi et al. (2015)
6 more

1 mention without citation

Structural basis for C-ribosylation in the alnumycin A biosynthetic pathway.
Oja et al. (2013)