› 4ewv

X-ray diffraction
2.9Å resolution

Crystal structure of GH3.12 in complex with AMPCPP

Released:
DOI: 10.2210/pdb4ewv/pdb
PDB entry 4ewv coloured by chain, front view.
PDB entry 4ewv coloured by chain, side view.
PDB entry 4ewv coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Structural basis for prereceptor modulation of plant hormones by GH3 proteins.
Westfall CS, Zubieta C, Herrmann J, Kapp U, Nanao MH, Jez JM
Science 336 1708-11 (2012)
PMID: 22628555

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192345 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
4-substituted benzoates-glutamate ligase GH3.12 Chains: A, B
Molecule details ›
Chains: A, B
Length: 581 amino acids
Theoretical weight: 65.76 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9LYU4 (Residues: 1-575; Coverage: 100%)
Gene names: At5g13320, GDG1, GH3.12, PBS3, T22N19.5, T31B5.140, WIN3
Sequence domains: GH3 auxin-responsive promoter

Ligands and Environments

2 bound ligands:
Ligand APC 2 x APC
Ligand MN 1 x MN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P41
Unit cell:
a: 116.348Å b: 116.348Å c: 94.663Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.246 0.243 0.286
Expression system: Escherichia coli

Quick links

Citations

15 review citations

Jasmonates: biosynthesis, perception, signal transduction and action in plant stress response, growth and development. An update to the 2007 review in Annals of Botany.
Wasternack et al. (2013)
14 more

3 mentions without citation

Enzyme action in the regulation of plant hormone responses.
Westfall et al. (2013)
2 more