› 4ena

X-ray diffraction
2.85Å resolution

Crystal structure of fluoride riboswitch, soaked in Cs+

Released:
DOI: 10.2210/pdb4ena/pdb
PDB entry 4ena coloured by chain, front view.
PDB entry 4ena coloured by chain, side view.
PDB entry 4ena coloured by chain, top view.
Source organism: Thermotoga petrophila
Primary publication:
Fluoride ion encapsulation by Mg2+ ions and phosphates in a fluoride riboswitch.
Ren A, Rajashankar KR, Patel DJ
Nature 486 85-9 (2012)
PMID: 22678284

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195908 (preferred)
Entry contents:
1 distinct RNA molecule
Macromolecule:
Fluoride riboswitch Chain: A
Molecule details ›
Chain: A
Length: 52 nucleotides
Theoretical weight: 16.94 KDa
Source organism: Thermotoga petrophila
Expression system: Not provided
Sequence domains: Fluoride riboswitch (crcB)

Ligands and Environments

3 bound ligands:
Ligand CS 5 x CS
Ligand MG 7 x MG
Ligand F 1 x F
1 modified residue:
Ligand GTP 1 x GTP

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P21212
Unit cell:
a: 58.742Å b: 77.149Å c: 42.477Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.229 0.225 0.264
Expression system: Not provided

Quick links

Citations

25 review citations

A decade of riboswitches.
Serganov et al. (2013)
24 more

3 mentions without citation

Fluoride ion encapsulation by Mg2+ ions and phosphates in a fluoride riboswitch.
Ren et al. (2012)
2 more