› 4ekv

X-ray diffraction
2Å resolution

Streptavidin 8-aa-loop H127C mutein with reversible biotin binding

Released:
DOI: 10.2210/pdb4ekv/pdb
PDB entry 4ekv coloured by chain, front view.
PDB entry 4ekv coloured by chain, side view.
PDB entry 4ekv coloured by chain, top view.
Source organism: Streptomyces avidinii
Primary publication:
Development of a tetrameric streptavidin mutein with reversible biotin binding capability: engineering a mobile loop as an exit door for biotin.
O'Sullivan VJ, Barrette-Ng I, Hommema E, Hermanson GT, Schofield M, Wu SC, Honetschlaeger C, Ng KK, Wong SL
PLoS One 7 e35203 (2012)
PMID: 22536357

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149694 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Streptavidin Chain: A
Molecule details ›
Chain: A
Length: 159 amino acids
Theoretical weight: 16.29 KDa
Source organism: Streptomyces avidinii
Expression system: Bacillus subtilis
UniProt:
  • Canonical: P22629 (Residues: 25-183; Coverage: 100%)
Sequence domains: Avidin family
Structure domains: Avidin-like

Ligands and Environments

2 bound ligands:
Ligand BTN 1 x BTN
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: P42212
Unit cell:
a: 57.066Å b: 57.066Å c: 70.186Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.188 0.225
Expression system: Bacillus subtilis

Quick links

Citations

2 review citations

Streptavidin-biotin technology: improvements and innovations in chemical and biological applications.
Dundas et al. (2013)
1 more