› 4eii

X-ray diffraction
1.95Å resolution

Unliganded E. cloacae R91K MurA

Released:
DOI: 10.2210/pdb4eii/pdb
PDB entry 4eii coloured by chain, front view.
PDB entry 4eii coloured by chain, side view.
PDB entry 4eii coloured by chain, top view.
Source organism: Enterobacter cloacae subsp. cloacae ATCC 13047
Entry authors: Han H, Zhu J-Y, Schonbrunn E

Function and Biology Details

Reaction catalysed: 
Phosphoenolpyruvate + UDP-N-acetyl-alpha-D-glucosamine = phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152534 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
UDP-N-acetylglucosamine 1-carboxyvinyltransferase Chain: A
Molecule details ›
Chain: A
Length: 419 amino acids
Theoretical weight: 44.8 KDa
Source organism: Enterobacter cloacae subsp. cloacae ATCC 13047
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P33038 (Residues: 1-419; Coverage: 100%)
Gene names: ECL_04571, murA, murZ
Sequence domains: EPSP synthase (3-phosphoshikimate 1-carboxyvinyltransferase)
Structure domains: Enolpyruvate transferase domain

Ligands and Environments

3 bound ligands:
Ligand GOL 2 x GOL
Ligand EDO 7 x EDO
Ligand PGE 1 x PGE
1 modified residue:
Ligand IAS 1 x IAS

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P212121
Unit cell:
a: 65.73Å b: 71.98Å c: 76.3Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.18 0.18 0.233
Expression system: Escherichia coli BL21(DE3)

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