› 4ehx

X-ray diffraction
1.9Å resolution

Crystal structure of LpxK from Aquifex aeolicus at 1.9 angstrom resolution

Released:
DOI: 10.2210/pdb4ehx/pdb
PDB entry 4ehx coloured by chain, front view.
PDB entry 4ehx coloured by chain, side view.
PDB entry 4ehx coloured by chain, top view.
Source organism: Aquifex aeolicus VF5
Primary publication:
Crystal structure of LpxK, the 4'-kinase of lipid A biosynthesis and atypical P-loop kinase functioning at the membrane interface.
Emptage RP, Daughtry KD, Pemble CW, Raetz CR
Proc Natl Acad Sci U S A 109 12956-61 (2012)
PMID: 22826246

Function and Biology Details

Reaction catalysed: 
ATP + (2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-beta-D-glucosaminyl)-(1->6)-(2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl phosphate) = ADP + (2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-4-O-phospho-beta-D-glucosaminyl)-(1->6)-(2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl phosphate)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130512 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tetraacyldisaccharide 4'-kinase Chain: A
Molecule details ›
Chain: A
Length: 315 amino acids
Theoretical weight: 36.6 KDa
Source organism: Aquifex aeolicus VF5
Expression system: Escherichia coli
UniProt:
  • Canonical: O67572 (Residues: 1-315; Coverage: 100%)
Gene names: aq_1656, lpxK
Sequence domains: Tetraacyldisaccharide-1-P 4'-kinase

Ligands and Environments

3 bound ligands:
Ligand EPE 1 x EPE
Ligand GOL 8 x GOL
Ligand MPD 2 x MPD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: P212121
Unit cell:
a: 61.783Å b: 69.049Å c: 106.079Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.174 0.172 0.2
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Biosynthesis and export of bacterial lipopolysaccharides.
Whitfield et al. (2014)
3 more

6 mentions without citation

Structure, inhibition, and regulation of essential lipid A enzymes.
Zhou et al. (2017)
5 more