› 4eec

X-ray diffraction
2.7Å resolution

Crystal Structure of the glycopeptide antibiotic sulfotransferase StaL complexed with A3P and desulfo-A47934.

Released:
DOI: 10.2210/pdb4eec/pdb
PDB entry 4eec coloured by chain, front view.
PDB entry 4eec coloured by chain, side view.
PDB entry 4eec coloured by chain, top view.
Source organism: Streptomyces toyocaensis
Primary publication:
Structural basis of sulfonation of the glycopeptide antibiotic scaffold by the sulfotransferase StaL
Shi R, Munger C, Kalan L, Sulea T, Wright GD, Cygler M
Proc. Natl. Acad. Sci. U.S.A. (2012)

Function and Biology Details

Reaction catalysed: 
3'-phosphoadenylyl sulfate + desulfo-A47934 = adenosine 3',5'-bisphosphate + A47934
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-164432 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Desulfo-A47934 sulfotransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 286 amino acids
Theoretical weight: 31.94 KDa
Source organism: Streptomyces toyocaensis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8KLM3 (Residues: 4-270; Coverage: 99%)
Gene names: BU52_01305, staL
Sequence domains: Sulfotransferase domain
Structure domains:
desulfo-A47934 Chain: C
Molecule details ›
Chain: C
Length: 7 amino acids
Theoretical weight: 1.24 KDa
Source organism: Streptomyces toyocaensis
Expression system: Streptomyces toyocaensis

Ligands and Environments

1 bound ligand:
Ligand A3P 1 x A3P
3 modified residues:
Ligand GHP 2 x GHP
Ligand 3MY 1 x 3MY
Ligand OMY 1 x OMY

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P212121
Unit cell:
a: 53.442Å b: 82.583Å c: 123.076Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.225 0.223 0.272
Expression systems:
  • Escherichia coli BL21(DE3)
  • Streptomyces toyocaensis

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