› 4e3y

X-ray diffraction
0.95Å resolution

X-ray structure of the Serratia marcescens endonuclease at 0.95 A resolution

Released:
DOI: 10.2210/pdb4e3y/pdb
PDB entry 4e3y coloured by chain, front view.
PDB entry 4e3y coloured by chain, side view.
PDB entry 4e3y coloured by chain, top view.
Source organism: Serratia marcescens
Entry authors: Lashkov AA, Balaev VV, Gabdoulkhakov AG, Betzel C, Mikhailov AM

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage to 5'-phosphomononucleotide and 5'-phosphooligonucleotide end-products
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146723 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nuclease Chains: A, B
Molecule details ›
Chains: A, B
Length: 245 amino acids
Theoretical weight: 26.74 KDa
Source organism: Serratia marcescens
UniProt:
  • Canonical: P13717 (Residues: 22-266; Coverage: 100%)
Gene names: nuc, nucA
Sequence domains: DNA/RNA non-specific endonuclease
Structure domains: Extracellular Endonuclease, subunit A

Ligands and Environments

5 bound ligands:
Ligand MG 2 x MG
Ligand EDO 2 x EDO
Ligand PEG 1 x PEG
Ligand SO4 2 x SO4
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7B
Spacegroup: P21212
Unit cell:
a: 106.265Å b: 73.418Å c: 67.924Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.131 0.13 0.145

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